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S3444
Sigma
Stemline® Dendritic Cell Maturation Medium
Serum-free, Without L -glutamine, liquid, sterile-filtered, cell culture tested
Description
| General description | Developed to promote the optimal propogation of mature Dendritic Cells (DC) from human peripheral blood CD14+ monocytes, Stemline Dendritic Cell Maturation Medium demonstrates superior numbers of mature dendritic cells when assessed both microscopically and by flow cytometry, when compared to alternative commercially available serum-free media. Additionally, the DC yield for Stemline is 3.4 to 5.5 times higher than that of other commercially avaiable media. Stemline Dendritic Cell Maturation Medium is free of serum and all other animal-derived components with the exceptions of human serum albumin, cholesterol, and transferrin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents. Produced in a GMP state-of-the-art facility with an available device master file (DMF), Stemline T Cell Expansion Medium is clearly an excellent choice for clinical applications. |
| Reconstitution | Medium must be supplemented with 2 mM L-glutamine, and with relevant growth factors and cytokines according to individual user protocols. |
| Legal Information | Stemline is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| sterility | sterile-filtered |
| form | liquid |
| quality | Drug/Device Master File available |
| suitability | cell culture tested |
| shipped in | wet ice |
| storage temp. | 2-8°C |
Safety
| Hazard Codes | B |
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References
| reference | Santos, K. et al, Infectivity of herpes simplex virus type-1 (HSV-1) amplicon vectors in dendritic cells is determined by the helper virus strain used for packaging J. Virol. Meth. 145, 37-46, (2007) Abstract |







